Morphometric measurements were performed in at least 25 individuals of each subspecies grown for 8—10 weeks under well-watered conditions. All plants were watered daily to field capacity until the start of the treatments. For assessing CAM plasticity, one-month-old plants were separated into three experimental groups subjected to different watering regimes: 1 well-watered, 2 droughted, and 3 rewatered.
Well-watered plants were continuously irrigated to field capacity throughout the experiment. Water was withheld from droughted and rewatered groups for 10 days, and subsequently, 10 mL water was added per pot whenever soil humidity reached values close to zero, usually every 4 days, for 20 consecutive days.
Plants of the rewatered group were irrigated to field capacity for the next 4 days. At the end of each treatment, samples were harvested 1 h after the onset of illumination dawn samples and 1 h before the end of the light period dusk samples. For all analyses, four biological replicates, each replicate composed of all fully-expanded and non-senescent leaves of at least three plants, were harvested at each sampling time.
Plants from all genotypes were grown side-by-side, and downstream analyses were performed with samples for all genotypes at the same time to avoid introducing unnecessary variation.
Subsequently, the leaf discs were fully-hydrated by incubation in deionized water for 24 h, followed by measuring the turgid weight TW.
Pellets were re-extracted three times, and all supernatants were combined and reduced to dryness under vacuum. Eluted compounds were detected at nm and quantified through external calibration. Endogenous metabolite concentrations were obtained by comparing the peak areas of the chromatograms against commercial standards.
The contribution of soil respiration and soil moisture to the environment in the cuvette was minimized by wrapping multiple-layers of parafilm around the rim of the pot, with a little hole for the stem. CO 2 exchange rates were based on leaf area, measured using ImageJ 1. Measurements were performed in at least two leaves from three different plants for each treatment.
Total RNA was extracted from approx. Purity was assessed by keeping ratios in between the following intervals: 1. In all cases, the melting curve was analyzed to detect unspecific amplification and primer dimerization. All primer sequences used are listed in Supplementary Table S7.
All statistical analyses were performed using R version 3. The data were checked for normality using the Shapiro—Wilk test, and for homogeneity of variances using the Levene test. Morphometric and climate data were normalized using z-scale and analyzed by principal component analysis PCA using the pre-installed R function prcomp visualized using factoextra package Hierarchical clustering analysis was done using the pvclust package version 2.
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The progressive development of the strategic programme of the Republic of Armenia for aims to increase the reliability and efficiency of the operation of the drinking water supply and sewage systems, reduce water losses and improve the quality of water and wastewater services.
Target 6. Similarly, target 6. Republic of the Armenia Water Code adopted on 4 June Sustainable Development Goals. UN Sustainable development goals, the sustainable.
Population not connected to water supply industry self-supply; estimation. Total household water use water supply industry and self-supply per capita. EEA, EEA core set of indicators guide. Questionnaire on Environment Statistics. Topics: water. Tags: water use water use per capita Armenia. Typology: descriptive indicator type A — what is happening to the environment and to humans? Ownership: Statistical Committee of the Republic of Armenia.
This Site Uses Cookies We may use cookies to record some preference settings and to analyse how you use our web site. C4 — Household water use per capita in the Republic of Armenia. Key messages An average Armenian citizen used 36 m 3 of water from renewable freshwater resources in compared with 48 m 3 in In , water use per capita on a daily basis was estimated at Figure 1 - Development of total freshwater use by households in the Republic of Armenia Chart Table.
Figure 2 - Development of household water use per capita in the Republic of Armenia Chart Table. Dates Publish date: Oct 31, Last modified: Nov 11, Frequency of updates: every year Related content: Short-term work Improve data on self-supply water use Long-term work No long-term work has been specified.
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